Physical Mapping Calculator - Library Construction
This very simple calculator shows how the library redundancy and %% of genome covered depends on the number of clones in a library. Redundancy (or the number of genome equivalents contained in a library) grows linearly with the number of clones and is limited by ten in this and Random Fingerprinting calculator. A proportion of genome covered (or a probability that a given sequence is found in a library) is known as Clarke and Carbon (1976) formula: 1-exp(-redundancy). Thanks to Jared Roach for suggesting me to include this calculator in the set and the original reference to Robbins (1944).
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Human Chromosomes
Approximate sizes of human chromosomes are given below (frankly, I don't remember where I've got this table from so if anything is wrong please let me know).
Chromosome | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | X | Y |
Size, Mb | 263 | 255 | 214 | 203 | 194 | 183 | 171 | 155 | 145 | 144 | 144 | 143 | 114 | 109 | 106 | 98 | 92 | 85 | 67 | 72 | 50 | 56 | 164 | 59 | 3286 |
References
Clarke, L. and Carbon, J. (1976) A colony bank containing synthetic ColE1 hybrid plasmids representative of the entire E. coli genome. Cell, 9, 91-101.
Robbins, H. E. (1944) On the measure of a random set. Ann. Math. Stat., 15, 70-74.
Comments are most welcome - just click on my name below to email me
Copyright 1996 Andrei Grigoriev
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