Physical Mapping Calculator - Library Construction

This very simple calculator shows how the library redundancy and %% of genome covered depends on the number of clones in a library. Redundancy (or the number of genome equivalents contained in a library) grows linearly with the number of clones and is limited by ten in this and Random Fingerprinting calculator. A proportion of genome covered (or a probability that a given sequence is found in a library) is known as Clarke and Carbon (1976) formula: 1-exp(-redundancy). Thanks to Jared Roach for suggesting me to include this calculator in the set and the original reference to Robbins (1944).

Pity you can't see this, your browser doesn't support Java applets.

Human Chromosomes

Approximate sizes of human chromosomes are given below (frankly, I don't remember where I've got this table from so if anything is wrong please let me know).

Chromosome12345678910111213141516171819202122XY
Size, Mb26325521420319418317115514514414414311410910698928567725056164593286

References

Clarke, L. and Carbon, J. (1976) A colony bank containing synthetic ColE1 hybrid plasmids representative of the entire E. coli genome. Cell, 9, 91-101.

Robbins, H. E. (1944) On the measure of a random set. Ann. Math. Stat., 15, 70-74.

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Copyright 1996 Andrei Grigoriev

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