Physical Mapping Calculator  Library Construction
This very simple calculator shows how the library redundancy and %% of genome covered depends on the number of clones in a library. Redundancy (or the number of genome equivalents contained in a library) grows linearly with the number of clones and is limited by ten in this and Random Fingerprinting calculator. A proportion of genome covered (or a probability that a given sequence is found in a library) is known as Clarke and Carbon (1976) formula: 1exp(redundancy). Thanks to Jared Roach for suggesting me to include this calculator in the set and the original reference to Robbins (1944).

Human Chromosomes
Approximate sizes of human chromosomes are given below (frankly, I don't remember where I've got this table from so if anything is wrong please let me know).
Chromosome  1  2  3  4  5  6  7  8  9  10  11  12  13  14  15  16  17  18  19  20  21  22  X  Y 
Size, Mb  263  255  214  203  194  183  171  155  145  144  144  143  114  109  106  98  92  85  67  72  50  56  164  59  3286 
References
Clarke, L. and Carbon, J. (1976) A colony bank containing synthetic ColE1 hybrid plasmids representative of the entire E. coli genome. Cell, 9, 91101.
Robbins, H. E. (1944) On the measure of a random set. Ann. Math. Stat., 15, 7074.
Comments are most welcome  just click on my name below to email me
Copyright 1996 Andrei Grigoriev
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