Two calculators are shown: one at the top is based on the (classic by now) paper by Lander and Waterman (1988), while that below - on a more recent publication of Roach (1995) and his personal communication. Their results are very similar for low library redundacy but start to differ at redundancies of three and higher, where the model of Jared Roach gives more consistent estimates (eg, for the contig length).
Another difference is in calculation of the expected number of gaps. Roach (1995) calculated the total number of all the gaps in the final map (which is the number of contigs plus one for a linear target), while Lander and Waterman (1988) provided results for "real" gaps (as opposed to undetected overlaps). You may need to enlarge your browser window or open a new window to see both calculators simulataneously.
These predictions are based on the following assumptions:
Such experimental factors as chimerism, repeats and false positive/negative results are ignored, so expect your project to progress slower than the ideal model :-(
Lander, E. and Waterman, M. S. (1988) Genomic mapping by fingerprinting random clones: a mathematical analysis. Genomics 2, 231-239.
Roach, J. C. (1995) Random Subcloning. Genome Research. 5, 464-473.
See also his web page on the subject.
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